CoMPLEX Fellowship Report - Samantha Lee

Location of Visit:

Montana State University, Bozeman MT, USA

Purpose of Visit:

Professor Thom Hughes is head of the Department of Neuroscience and Cell Biology at Montana State University.  He pioneered the use of the green fluorescent protein, demonstrating for the first time that it could provide a marker for transfection and that it could also be used to tag non-fluorescent proteins, such as ion channels, without disrupting their function. These techniques are now very widely applied in laboratories around the world.  One of his current major interests is in the development of novel fluorescent biosensors. To aid with their rapid development he has invented a number of constructs that combine Tn5 transposon technologies with fluorescent proteins to quickly generate libraries of fluorescently labelled proteins. During my visit to the Hughes lab I used this technique to aid in the creation of a FRET-based sensor of peptide secretion. The Hughes lab was the ideal place for me to learn this technique. It was also a great opportunity for me to visit a lab with such a strong background in the design and creation of fluorescent biosensors.


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