CoMPLEX Fellowship Report - Julija Krupic

Location of Visit:

University of Zurich, Zurich, Switzerland

Purpose of Visit:
Tetrode recording is a widely used technique for in vivo chronic recordings of extracellular currents associated with action potentials. It is easy to apply in various behavioural tasks, it provides a good signal-to-noise ratio (SNR) and enables reliable recordings of many neurons at the same time. The major drawback of this method is that it does not provide the information about the anatomical arrangement of the recorded cells and their connectivity. Identifying the exact anatomical location of a particular cell would permit the correlation of its functional properties with neural type determined by immunostaining. The aim of my project in Prof. Fritjof Helmchen’s lab in the University of Zurich was therefore to combine Ca2+ imaging with tetrode recordings to record in vivo neural activity in the rat barrel cortex. To our knowledge, this has not been accomplished before.

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