Force probing living cells to molecular resolution

Daniel J. Müller, Professor of Cellular Machines, Biotechnology Center, Dresden

Tuesday 28 April, Location: Cruciform LT2, time: 16:30

Biological processes rely on molecular interactions that can be directly measured using force spectroscopy techniques. I will review how atomic force microscopy (AFM) can be applied to force probe living cells to single-molecule resolution. Such probing of individual interactions can be used to map cell surface receptors, and to assay the receptors functional state, binding kinetics and landscape. However, AFM can be also used to image the cellular machinery at work and at a resolution approaching 1 nm. In combination both approaches provide a unique toolbox that allows to image and to probe the molecular machinery of the cell at work.

Key words: ATP synthases, gap junctions, GPCRs, transporters, microtubuli, membrane proteins, ligand- and inhibitor binding, cell sorting, cell adhesion, energy landscape

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Muller Poster

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