Reverse engineering of proteasomal translocation rates

Alexey Zaikin, University College London

Tuesday 30th June, Location: Cruciform LT2, Time: 16-30

Proteasomes are fundamental for the degradation of intracellular proteins, having a key role in several important metabolic and signalling pathways, in the cell cycle and in antigen presentation. Though proteasomes are important for all aspects of the cellular metabolism,  and are  the main compartments of the immune system, details of the physical mechanism of the proteasomal translocation and cleavage process remain unknown. We address here the problem of proteasomal protein transport and introduce a new stochastic model, which accounts for the protein translocation and the topology of the positioning of cleavage centers of a proteasome from first principles.

We show by test examples and by a comparison with experimental data that our model allows reconstruction of the translocation rates from mass spectroscopy data on digestion patterns. The proposed methodology can be used in extensive analysis of already available MS data for the 20S proteasomes and its associations with different regulatory complexes and under different experimental conditions, hence, giving insight into the mystery of the protein translocation and cleavage mechanism inside the proteasome.

Please contact Raphaela Heussen ( if you would like to meet the speaker.

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