Dr Stefan Howorka - Publications


    • Buchsbaum, S. F., Nguyen, G., Howorka, S., Siwy, Z. S. (2014). DNA-Modified Polymer Pores Allow pH- and Voltage-Gated Control of Channel Flux. J Am Chem Soc doi:10.1021/ja505302q. Author URL
    • Goyal, P., Krasteva, P. V., Van Gerven, N., Gubellini, F., Van den Broeck, I., Troupiotis-Tsaïlaki, A., Jonckheere, W., Péhau-Arnaudet, G., Pinkner, J. S., Chapman, M. R., Hultgren, S. J., Howorka, S., Fronzes, R., Remaut, H. (2014). Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG. Nature doi:10.1038/nature13768. Author URL
    • Howorka, S., Hesse, J. (2014). Microarrays and single molecules: an exciting combination. SOFT MATTER 10(7), 931-941 doi:10.1039/c3sm52561a. Author URL


    • Buchsbaum, S. F., Mitchell, N., Martin, H., Wiggin, M., Marziali, A., Coveney, P. V., Siwy, Z., Howorka, S. (2013). Disentangling Steric and Electrostatic Factors in Nanoscale Transport Through Confined Space. Nano Lett doi:10.1021/nl401968r. Author URL
    • Burns, J. R., Göpfrich, K., Wood, J. W., Thacker, V. V., Stulz, E., Keyser, U. F., Howorka, S. (2013). Lipid-Bilayer-Spanning DNA Nanopores with a Bifunctional Porphyrin Anchor. Angew. Chem. Int. Ed. , n/a-n/a doi:10.1002/anie.201305765. Author URL
    • Burns, J. R., Stulz, E., Howorka, S. (2013). Self-Assembled DNA Nanopores That Span Lipid Bilayers. Nano Lett 13(6), 2351-2356 doi:10.1021/nl304147f. Author URL
    • Howorka, S. (2013). DNA nanoarchitectonics: assembled DNA at interfaces. Langmuir 29(24), 7344-7353 doi:10.1021/la3045785. Author URL


    • Baranova, E., Fronzes, R., Garcia-Pino, A., Van Gerven, N., Papapostolou, D., Péhau-Arnaudet, G., Pardon, E., Steyaert, J., Howorka, S., Remaut, H. (2012). SbsB structure and lattice reconstruction unveil Ca2+ triggered S-layer assembly. Nature 487(7405), 119-122 doi:10.1038/nature11155. Author URL
    • Howorka, S., Siwy, Z. S. (2012). Nanopores as protein sensors. Nat Biotechnol 30(6), 506-507 doi:10.1038/nbt.2264. Author URL
    • Schlapak, R., Danzberger, J., Armitage, D., Morgan, D., Ebner, A., Hinterdorfer, P., Pollheimer, P., Gruber, H. J., Schäffler, F., Howorka, S. (2012). Nanoscale DNA Tetrahedra Improve Biomolecular Recognition on Patterned Surfaces. Small 8(1), 89-97 doi:10.1002/smll.201101576. Author URL
    • Schlapak, R., Danzberger, J., Haselgrübler, T., Hinterdorfer, P., Schäffler, F., Howorka, S. (2012). Painting with Biomolecules at the Nanoscale: Biofunctionalization with Tunable Surface Densities. Nano Lett 12(4), 1983-1989 doi:10.1021/nl2045414. Author URL


    • Howorka, S. (2011). Molecular assembly in natural and engineered systems. Preface. Prog Mol Biol Transl Sci 103, xi-xii doi:10.1016/B978-0-12-415906-8.00014-5. Author URL
    • Howorka, S. (2011). Rationally engineering natural protein assemblies in nanobiotechnology. Curr Opin Biotechnol 22(4), 485-491 doi:10.1016/j.copbio.2011.05.003. Author URL
    • Leitner, M., Mitchell, N., Kastner, M., Schlapak, R., Gruber, H. J., Hinterdorfer, P., Howorka, S., Ebner, A. (2011). Single-Molecule AFM Characterization of Individual Chemically Tagged DNA Tetrahedra. ACS Nano 5(9), 7048-7054 doi:10.1021/nn201705p. Author URL
    • Nguyen, G., Howorka, S., Siwy, Z. S. (2011). DNA Strands Attached Inside Single Conical Nanopores: Ionic Pore Characteristics and Insight into DNA Biophysics. J MEMBR BIOL 239(1-2), 105-113 doi:10.1007/s00232-010-9328-4.
    • Pavkov-Keller, T., Howorka, S., Keller, W. (2011). The Structure of Bacterial S-Layer Proteins. Prog Mol Biol Transl Sci 103, 73-130 doi:10.1016/B978-0-12-415906-8.00004-2. Author URL


    • Caruana, D. J., Howorka, S. (2010). Biosensors and biofuel cells with engineered proteins. MOL BIOSYS 6(9), 1548-1556 doi:10.1039/c004951d.
    • Kinns, H., Badelt-Lichtblau, H., Egelseer, E. M., Sleytr, U. B., Howorka, S. (2010). Identifying Assembly-Inhibiting and Assembly-Tolerant Sites in the SbsB S-Layer Protein from Geobacillus stearothermophilus. J MOL BIOL 395, 742-753 doi:10.1016/j.jmb.2009.10.012.
    • Kukwikila, M., Howorka, S. (2010). Electrically sensing protease activity with nanopores. J PHYS-CONDENS MAT 22(45), 454103 doi:10.1088/0953-8984/22/45/454103.
    • Leung, C., Maradan, D., Kramer, A., Howorka, S., Mesquida, P., Hoogenboom, B. W. (2010). Improved Kelvin probe force microscopy for imaging individual DNA molecules on insulating surfaces. APPL PHYS LETT 97(20), 203703 doi:10.1063/1.3512867.
    • Mitchell, N., Ebner, A., Hinterdorfer, P., Tampe, R., Howorka, S. (2010). Chemical Tags Mediate the Orthogonal Self-Assembly of DNA Duplexes into Supramolecular Structures. SMALL 6(16), 1732-1735 doi:10.1002/smll.201000790.
    • Winroth, G., Fenwick, O., Scott, M. A., Yip, D., Howorka, S., Cacialli, F. (2010). Interfacial dipole dynamics of light-emitting diodes incorporating a poly(amidoamine) dendrimer monolayer. APPL PHYS LETT 97(4), 043304 doi:10.1063/1.3459964.
    • Zhu, R., Howorka, S., Proll, J., Kienberger, F., Preiner, J., Hesse, J., Ebner, A., Pastushenko, V. P., Gruber, H. J., Hinterdorfer, P. (2010). Nanomechanical recognition measurements of individual DNA molecules reveal epigenetic methylation patterns. NAT NANOTECHNOL 5(11), 788-791 doi:10.1038/nnano.2010.212.
    • Ziwy, S., Howorka, S. (2010). Engineered voltage-responsive nanopores. CHEM SOC REV 39(3), 1115-1132 doi:10.1039/b909105j.


    • Borsenberger, V., Howorka, S. (2009). Diene-modified nucleotides for the DielsAlder-mediated functional tagging of DNA. NUCLEIC ACIDS RES 37(5), 1477-1485 doi:10.1093/nar/gkn1066.
    • Borsenberger, V., Kukwikila, M., Howorka, S. (2009). Synthesis and enzymatic incorporation of modified deoxyuridine triphosphates. ORG BIOMOL CHEM 7(18), 3826-3835 doi:10.1039/b906956a.
    • Borsenberger, V., Mitchell, N., Howorka, S. (2009). Chemically Labeled Nucleotides and Oligonucleotides Encode DNA for Sensing with Nanopores. J AM CHEM SOC 131(22), 7530-7531 doi:10.1021/ja902004s.
    • Howorka, S., Siwy, Z. (2009). Nanopores: Generation, engineering and single-molecule applications. In Handbook of Single-Molecule Biophysics ( pp.293-340). New York Springer.
    • Howorka, S., Ziwy, S. (2009). Nanopore analytics: sensing of single molecules. CHEM SOC REV 38(8), 2360-2384 doi:10.1039/b813796j.
    • Leung, C., Kinns, H., Hoogenboom, B., Howorka, S., Mesquida, P. (2009). Imaging Surface Charges of Individual Biomolecules. NANOLETT 9(7), 2769-2773 doi:10.1021/nl9012979.
    • Martin, H., Jha, S., Howorka, S., Coveney, P. (2009). Determination of Free Energy Profiles for the Translocation of Polynucleotides through alpha-Hemolysin Nanopores using Non-Equilibrium Molecular Dynamics Simulations. J CHEM THEORY COMPUT 5(8), 1955-2192 doi:10.1021/ct9000894.
    • Mitchell, N., Schlapak, R., Kastner, M., Armitage, D., Chrzanowski, W., Riener, J., Hinterdorfer, P., Ebner, A., Howorka, S. (2009). A DNA Nanostructure for the Functional Assembly of Chemical Groups with Tunable Stoichiometry and Defined Nanoscale Geometry. ANGEW CHEM INT ED 48(3), 525-527 doi:10.1002/anie.200804264.
    • Papapostolou, D., Howorka, S. (2009). Engineering and exploiting protein assemblies in synthetic biology. MOL BIOSYS 5(7), 723-732 doi:10.1039/b902440a.
    • Pollheimer, P. D., Kastner, M., Ebner, A., Blaas, D., Hinterdorfer, P., Gruber, H. J., Howorka, S. (2009). Receptor Arrays for the Selective and Efficient Capturing of Viral Particles. BIOCONJUGATE CHEM 20(3), 466-475 doi:10.1021/bc800357j.
    • Schlapak, R., Armitage, D., Saucedo-Zeni, N., Chrzanowski, W., Hohage, M., Caruana, D., Howorka, S. (2009). Selective protein and DNA adsorption on PLL-PEG films modulated by ionic strength. SOFT MATTER 5(3), 613-621 doi:10.1039/b815065f.
    • Schlapak, R., Caruana, D., Armitage, D., Howorka, S. (2009). Semipermeable poly(ethylene glycol) films: the relationship between permeability and molecular structure of polymer chains. SOFT MATTER 5(21), 4104-4112 doi:10.1039/b913836f.
    • Zhu, R., Ebner, A., Kastner, M., Preiner, J., Howorka, S., Hinterdorfer, P. (2009). Topography and Recognition Imaging of Protein-Patterned Surfaces Generated by AFM Nanolithography. CHEMPHYSCHEM 10(9-10), 1478-1481 doi:10.1002/cphc.200900245.


    • Artelsmair, H., Kienberger, F., Tinazli, A., Schlapak, R., Preiner, J., Wruss, J., Kastner, M., Saucedo-Zeni, N., Hoelzl, M., C, R., C, B., S, H., S, B., D, G., H J, T., R, H. (2008). Atomic force microscopy-derived nanoscale chip for the detection of human pathogenic viruses. SMALL 4(6), 847-854 doi:10.1002/smll.200700691.
    • Kinns, H., Howorka, S. (2008). The surface location of individual residues in a bacterial S-layer protein. J MOL BIOL 377(2), 589-604 doi:10.1016/j.jmb.2008.01.019.
    • Latini, G., Wykes, M., Schlapak, R., Howorka, S., Cacialli, F. (2008). Self-assembled monolayers of protonated poly(amidoamine) dendrimers on indium tin oxide. APPL PHYS LETT 92(1), 013511- doi:10.1063/1.2829387.
    • Mitchell, N., Howorka, S. (2008). Chemical tags facilitate the sensing of individual DNA strands with nanopores. ANGEW CHEM INT ED 47(30), 5565-5568 doi:10.1002/anie.200800183.


    • Astier, Y., Kainov, D. E., Bayley, H., Tuma, R., Howorka, S. (2007). Stochastic detection of motor protein-RNA complexes by single-channel current recording. CHEMPHYSCHEM 8(15), 2189-2194 doi:10.1002/cphc.200700179.
    • Drbal, K., Moertelmaier, M., Holzhauser, C., Muhammad, A., Fuertbauer, E., Howorka, S., Hinterberger, M., Stockinger, H., Schutz, G. J. (2007). Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. INT IMMUNOL 19(5), 675-684 doi:10.1093/intimm/dxm032.
    • Howorka, S. (2007). Creating regular arrays of nanoparticles with self-assembling protein building blocks. J MATER CHEM 17(20), 2049-2053 doi:10.1039/b701221g.
    • Martin, H., Kinns, H., Mitchell, N., Astier, Y., Madathil, R., Howorka, S. (2007). Nanoscale protein pores modified with PAMAM dendrimers. J AM CHEM SOC 129(31), 9640-9649 doi:10.1021/ja0689029.
    • Schlapak, R., Armitage, D., Saucedo-Zeni, N., Hohage, M., Howorka, S. (2007). Dense passivating poly(ethylene glycol) films on indium tin oxide substrates. LANGMUIR 23(20), 10244-10253 doi:10.1021/la7011414.
    • Schlapak, R., Armitage, D., Saucedo-Zeni, N., Latini, G., Gruber, H. J., Mesquida, P., Samotskaya, Y., Hohage, M., Cacialli, F., Howorka, S. (2007). Preparation and characterization of dense films of poly(amidoamine) dendrimers on indium tin oxide. LANGMUIR 23(17), 8916-8924 doi:10.1021/la700476w.
    • Schlapak, R., Kinns, H., Wechselberger, C., Hesse, J., Howorka, S. (2007). Sizing trinucleotide repeat sequences by single-molecule analysis of fluorescence brightness. CHEMPHYSCHEM 8(11), 1618-1621 doi:10.1002/cphc.200700163.


    • Hesse, J., Jacak, J., Kasper, M., Regl, G., Eichberger, T., Winklmayr, M., Aberger, F., Sonnleitner, M., Schlapak, R., Howorka, S., Muresan, L., Frischauf, A. M., Schutz, G. J. (2006). RNA expression profiling at the single molecule level. GENOME RES 16(8), 1041-1045 doi:10.1101/gr.4999906.
    • Schlapak, R., Pammer, P., Armitage, D., Zhu, R., Hinterdorfer, P., Vaupel, M., Fruhwirth, T., Howorka, S. (2006). Glass surfaces grafted with high-density poly(ethylene glycol) as substrates for DNA oligonucleotide microarrays. LANGMUIR 22(1), 277-285 doi:10.1021/la0521793.


    • Astier, Y., Bayley, H., Howorka, S. (2005). Protein components for nanodevices. CURR OPIN CHEM BIOL 9(6), 576-584 doi:10.1016/j.cbpa.2005.10.012.
    • Pammer, P., Schlapak, R., Sonnleitner, M., Ebner, A., Zhu, R., Hinterdorfer, P., Höglinger, O., Schindler, H. G., Howorka, S. (2005). Nanopatterning of biomolecules with microscale beads. CHEMPHYSCHEM 6, 900-903 doi:10.1002/cphc.200400526.


    • Howorka, S., Nam, J., Bayley, H., Kahne, D. (2004). Stochastic detection of monovalent and bivalent protein-ligand interactions. ANGEW CHEM INT EDIT 43(7), 842-846 doi:10.1002/anie.200352614.


    • Howorka, S., Bayley, H. (2002). High-throughput scanning mutagenesis by recombination PCR. In Braman, J. (Ed.). In vitro Mutagenesis Protocols ( pp.139-147). Totowa, NJ Humana Press.
    • Howorka, S., Bayley, H. (2002). High-throughput scanning mutagenesis by recombination polymerase chain reaction. METHODS MOL BIOL 182, 139-147
    • Howorka, S., Bayley, H. (2002). Probing distance and electrical potential within a protein pore with tethered DNA. BIOPHYS J 83(6), 3202-3210
    • Howorka, S., Cheley, S., Movileanu, L., Braha, O., Bayley, H. (2002). DNA duplex formation of individual DNA strands within a single protein pore. BIOPHYS J 82(1), 508A-508A


    • Howorka, S., Cheley, S., Bayley, H. (2001). Sequence-specific detection of individual DNA strands using engineered nanopores. NAT BIOTECHNOL 19(7), 636-639 doi:10.1038/90236.
    • Howorka, S., Movileanu, L., Braha, O., Bayley, H. (2001). Kinetics of duplex formation for individual DNA strands within a single protein nanopore. PROC NATL ACAD SCI USA 98(23), 12996-13001 doi:10.1073/pnas.231434698.
    • Movileanu, L., Cheley, S., Howorka, S., Braha, O., Bayley, H. (2001). Location of a constriction in the lumen of a transmembrane pore by targeted covalent attachment of polymer molecules. J GEN PHYSIOL 117(3), 239-252 doi:10.1085/jgp.117.3.239.


    • Howorka, S., Movileanu, L., Lu, X., Magnon, M., Cheley, S., Braha, O., Bayley, H. (2000). A protein pore with a single polymer chain tethered within the lumen. J AM CHEM SOC 122(11), 2411-2416 doi:10.1021/ja993221h.
    • Howorka, S., Sára, M., Wang, Y., Kuen, B., Sleytr, U. B., Lubitz, W., Bayley, H. (2000). Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. J BIOL CHEM 275(48), 37876-37886 doi:10.1074/jbc.M003838200.
    • Movileanu, L., Howorka, S., Braha, O., Bayley, H. (2000). Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore. NAT BIOTECHNOL 18(10), 1091-1095 doi:10.1038/80295.


    • Howorka, S., Sara, M., Lubitz, W., Kuen, B. (1999). Self-assembly product formation of the Bacillus stearothermophilus PV72/p6 S-layer protein SbsA in the course of autolysis of Bacillus subtilis. FEMS MICROBIOL LETT 172(2), 187-196 doi:10.1016/S0378-1097(99)00040-3.


    • Howorka, S., Bayley, H. (1998). Improved protocol for high-throughput cysteine scanning mutagenesis. BIOTECHNIQUES 25(5), 764-772 Author URL


    • Truppe, M., Howorka, S., Schroll, G., Lechleitner, S., Resch, S., Kuen, B., Lubitz, W. (1997). Biotechnological applications of recombinant S-layer proteins rSbsA and rSbsB from B. stearothermophilus PV72. FEMS MICROBIOL REV 20(1-2), 88-98


    • Breitwieser, A., Kupcu, S., Howorka, S., Weigert, S., Langer, C., Hoffmann Sommergruber, K., Scheiner, O., Sleytr, U. B., Sára, M. (1996). 2-D protein crystals as an immobilization matrix for producing reaction zones in dipstick-style immunoassays. BIOTECHNIQUES 21(5), 918-925 Author URL