4th April 2016
20 places available

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Worth 6 Roberts points

Programme 2015

All practicals in Cruciform Teaching wet lab 2

Each practical session will start with a short Power Point introduction about the topic, basic principles of techniques/methodologies employed and the expected outcome of experimental set-ups.

Day 1

9:00 - 9:15
Registration and welcome pack - ground floor Cruciform foyer
9:15 - 10:00
Cruciform foyer 101 Seminar Room 1
Welcome address from the organisers and coffee and pastries to be served
10:00 - 13:00

Practical session 1:

Part 1 Culturing and maintenance of mammalian cells (monkey kidney fibroblast cell line COS-7).

The experience will be gained in splitting and counting cells, setting out sterility tests etc. The techniques of growing cells on the substrate (COS7) will be taught.

Part 2 Cryopreservation of mammalian cells.

Part 3 Setting up sterility tests

13:00 - 14:30
Lunch  (Cruciform Interactive cafe)
14:30 - 15:30
Cruciform foyer 101  Seminar Room 1
Lecture 1:
  Professor David Gems
"Understanding the Biology of Ageing"

15:30 - 18:00

Practical session 2:

Part 1 Transient transfection of COS cells.

Transient transfection of COS cells with pEGFP-N1/GFP and pEGFP-N1/GFP-CoA synthase using TurboFect transfection reagent (Fermentas)

Part 2 Analysis and application of mammalian cell lines.

During this practical session, the participants will have the opportunity to observe under the light microscope a range of mammalian cell lines, including human embryonic stem cells (HEK293), macrophages, pluripotent stem cells. Specific examples of their application in academic research and the development of therapeutic approaches will be discussed.

 18:30 - 21:00

Anatomy Student Hub UCL

Drinks and food to be served  

Day 2

9:00 - 10:00

Rockefeller G02

Lecture 2  Professor Geraint Thomas

"Interactions between cells and their Environments:  general principles in cell signalling and signal transduction"

 10:00 - 10:30
 Coffee break and discussion
10:30 - 13:00

Practical Session 3:

Part 1 Treatment of cells with Rapamycin (mTOR kinase inhibitor, cytostatic), doxorubicin (DNA intercalating drug, cytotoxic), Wortmannin (PI3K inhibitor, pro-apoptotic, cytotoxic). The effect of tested drugs will be examined in the CellTiter Cell Proliferation Assay. Discussion of the involvement of signal transduction pathways in the regulation of cell growth, proliferation and survival in mammalian cells.

Part 2 Revival of cryopreserved mammalian cells

13:00 - 14:30
Rockefeller G02
New! - Working Lunch

Carla Milagre - Research Associate at Magnus Life Science will give a talk.  Her expertise is in oncology and cancer biology
Coffee, tea and sandwiches provided
14:30 - 15:30

Rockefeller GO2

Lecture 3: Professor Jeremy Brockes

"Cell biology underlying vertebrate limb regeneration"

15:30 - 18:00

Cruciform Dry Lab 3

Tutorial 1: Professor G. Thomas and Professor I. Gout
The objective of this practical tutorial is to gain an appreciation of the nature of the digital immunofluorescence microscopy image and to experiment with semi-quantification.  It will be carried out in the computer cluster room.  The participants will have the opportunity to gain the experience in using various computer programs in order to compare the intensity of immunofluorescent signals.

Day 3

 9:00 - 10:00

Cruciform foyer 101  Seminar Room 1

Lecture 4:  Ms. Ludmila Ruban
"Human Pluripotent Stem Cell in Culture"

  coffee break and discussion
10:30 - 13:00

Practical Session 4:

This session is sponsored and organised by Thermo Fisher Scientific.

Part 1 Testing cell viability and proliferation by the CellTiter Cell Proliferation Assay (initiation of the assay).

Part 2 Examining the expression of GFP and GFP/CoA synthase fusion proteins in transfected cells by immunofluorescent microscopy.

The participants will compare the transfection efficiency and subcellular localization of expressed proteins.

Part 3 Examination and splitting of revived COS7 cells (Session 3, part 2).

13:00 - 14:30
14:30 - 15:30

Cruciform Foyer 101 Seminar Room 1

Lecture 5:  Professor Ivan Gout
"The control of cellular metabolism and growth by signalling pathways"    

15:30 - 18:00

Practical Session 5:

Part 1 Completion and analysis of the Cell Proliferation Assay

The participants will assess the viability and proliferation of cells grown in the presence or absence of Rapamycin (mTOR kinase inhibitor), doxorubicin (cytotoxic, DNA intercalating drug) and Wortmannin (PI3K inhibitor) using the MTT Cell Proliferation Assay. It is a colorimetric assay which offers a quantitative, convenient method for evaluating cellular response to diverse modulators of cell growth and survival.

Part 2 Summing up tutorial 2. The participants will have the opportunity to discuss the application of acquired techniques in experimental set-ups relevant to their research projects.

Presentation of course certificates to participants