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Deadline:
1st June
Full
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Worth 6 Roberts points

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Programme 2014

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All lectures will take place in Cruciform Foyer 101 Seminar Room 1

All practicals in Cruciform Teaching wet lab G2.01

Tutorial 1 and 2 will take place in DMS Watson G15

Each practical session will start with a short Power Point introduction about the topic, basic principles of techniques/methodologies employed and the expected outcome of experimental set-ups.

Day 1

 
9:00 - 9:15
Registration and welcome pack - Cruciform Foyer
9:15 - 10:00
Welcome address from the organisers and coffee to be served
10:00 - 13:00

Practical Session 1

Part1:  Culturing and maintenance of mammalian cells (monkey kidney fibroblast cell line COS-7).
During this practical session, the participants will have the opportunity to observe under the light microscope different types of mammalian cells, including COS7, HEK293 (human embryonic kidney 293), MCF7 (breast cancer cell line) and mouse embryonic stem cells.  The experience will be gained in splitting and counting COS7 cells, setting out sterility tests etc.  The techniques of growing cells on the substrate will be taught.
Part 2:  Cryopreservation of mammalian cells

13:00 - 14:30
Lunch  (Cruciform Interactive cafe)
14:30 - 15:30
Lecture 1:  Professor David Saggerson
"Studying Metabolism in Mammalian Cells"
15:30 - 18:00

Practical Session 2

This session is sponsored and organised by Miltenyi Biotec
Part 1:  Transient transfection of COS7 cells with plasmid DNA.  Transient transfection of HEK293 cells with pEGFP-N1/GFP and pEGEP-N1/GFP-CoA synthase using TurboFect transfection reagent (Fermentas).
Part 2:  Live cell staining of mouse embryonic stem cells

 18:30 - 21:00

Reception in Anatomy Student Hub UCL

Drinks and canapes to be served  

Day 2

 
9:00 - 10:00

Lecture 2  Dr L. Ruban

"Human pluripotent stem cell in culture"

 10:00 - 10:30
 Coffee break and discussion
10:30 - 13:00

Practical Session 3

PART 1:  Treatment of COS7 cells with Rapamycin (mTOR kinase inhibitor, cytostatic), doxorubicin (DNA intercalating drug, cytotoxic), TNFalpha (pro-apoptotic cytokine).  The effect of tested drugs/cytokine will be examined in the CellTiter Cell Proliferation Assay.
PART2:  Revival of cryopreserved mammalian cells

13:00 - 14:30
Lunch
14:30 - 15:30

Lecture 3: Professor J. Brockes

"Cell biology underlying vertebrate limb regeneration"

15:30 - 18:00
Tutorial 1: Professor G. Thomas and Professor I. Gout
The objective of this practical tutorial is to gain an appreciation of the nature of the digital immunofluorescence microscopy image and to experiment with semi-quantification.  It will be carried out in the computer cluster room.  The participants will have the opportunity to gain the experience in using various computer programs in order to compare the intensity of immunofluorescent signals.

Day 3

 
 9:00 - 10:00
Lecture 4:  Professor G. Thomas
"Interactions between cells and their environments: general principles in cell signalling & signal transduction"
10:00 - 10:30
Coffee break and discussion
10:30 - 13:00

Practical Session 4

This session is sponsored and organised by
Thermo Fisher Scientific
PART 1:
  Testing cell viability and proliferation by the CellTiter Cell Proliferation Assay (initiation of the assay)
PART 2:  Examining the expression of GFP and GFP/CoA synthase fusion proteins in transfected cells by immunofluorescent microscopy.  The participants will compare the transfection efficiency and subcellular localisation of expressed proteins.

13:00 - 14:30
Lunch
14:30 - 15:30
Lecture 5:  Professor I. Gout
"The control of cellular metabolism and growth by signalling pathways"                                            
15:30 - 18:00

Practical Session 5

PART 1.  Completion and analysis of the Cell Proliferation Assay.
The participants will assess the viability and proliferation of cells grown in the presence or absence of Rapamycin (mTOR kinase inhibitor), doxorubicin (cytotoxic, DNA intercalating drug), TNF alpha (tumor necrosis factor-alpha, cytokine) suing the MTT Cell Proliferation Assay.  It is a colormetric assay which offers a quantitative, convenient method for evaluating cellular response to diverse modulators of cell growth and survival.
PART 2  Summing up tutorial.  The participants will have the opportunity to discuss the application of acquired techniques in experimental set-ups relevant to their research projects.


Presentation of course certificates to participants