BiosciencesCPD

Deadline:
4th April 2015
20 places available

Apply here


Worth 6 Roberts points


Sponsored by

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Course Details

The Practicals will offer hands-on experience in:

  • Good cell culture practice, including safety procedures, control of facilities, equipment, reagents
  • Qualitative characteristics of mammalian cell cultures:  cell counting and analysis
  • Cryopreservation and cell banking
  • Sterility and microbial contamination tests
  • Maintenance and manipulation of stem cells, primary and established cell lines under various experimental conditions (mitogenic stimulation, exposure to cellular stresses and major signal transduction inhibitors)
  • Transient transfection of mammalian cells, expressing wild type and mutant proteins
  • Immunofluroescent analysis of subcellualr localisation of transiently expressed proteins
  • Quantitative analysis of immunofluorescence images by computer software
  • Assess the viability and proliferation of cells grown under various experimental conditions using the MTT Cell Proliferation Assay
  • Discussion and trouble-shooting

Course Lectures will focus on the following topics:

  • Whole tissue/cell techniques (immunofluorescence and immunoelectron microscopy, subcellualr fractionation, FACS analysis etc)
  • The control of cellular metabolism and growth by signalling pathways
  • Interactions between cells and their environments: general principles in cell signalling and signal transduction
  • Regulation of cell survival and proliferation in mammalian cells
  • Cell biology underlying vertebrate limb regeneration
  • Stem cell biology in health and disease
  • Human pluripotent stem cells in culture