Lab Techniques in Mammalian Cell Biology
Short Courses from the Division of Biosciences
Latest News
Miltenyi Biotec have agreed to run a practical session on:
- Live cell staining of HEK293 and mouse embryonic stem cells
- Transient transfection of HEK293 cells with plasmid DNA and eGFP mRNA
University College London 8 - 10 July 2013
Extensive hands-on-training and lectures
Whether you are a complete beginner with little or no experience of mammalian cell culture, or would like to extend your existing knowledge and enhance your professional profile, this theoretical and laboratory-based training course can meet your requirements.
Practical Course will offer hands-on experience in:
- Good cell culture practice, including safety procedures, control of facilities, equipment, reagents
- Qualitative characteristics of mammalian cell cultures: cell counting and analysis
- Cryopreservation and cell banking
- Sterility and microbial contamination tests, including Mycoplasma screening
- Maintenance and manipulation of stem cells, primary and established cell lines under various experimental conditions (mitogenic stimulation, exposure to cellular stresses and major signal transduction inhibitors)
- Transient transfection of mammalian cell lines, expressing wild type and mutant proteins
- Knock down the expression of cellular proteins by lentiviral shRNAs
- Immunofluroescent analysis of subcellualr localisation of transiently expressed proteins
- Quantitative analysis of immunofluorescence microscopy images using computer software
- Quantitative analysis of immunofluorescence images by computer software
- Assess the viability and proliferation of cells grown under various experimental conditions using the MTT Cell Proliferation Assay
- Discussion and trouble-shooting
Course Lectures will focus on the following topics:
- Whole tissue/cell techniques (immunofluorescence and immunoelectron microscopy, subcellualr fractionation, FACSW analysis etc)
- The control of cellular metabolism and growth by signalling pathways
- Interactions between cells and their environments: general principles in cell signalling and signal transduction
- Regulation of cell survival and proliferation in mammalian cells
- Cell biology underlying vertebrate limb regeneration
- Stem cell biology in health and disease
- Application of confocal, time-lapse and 2-photon microscopy in mammalian cell biology
