All Seminars are held in the Gavin De Beer Lecture Theatre, Anatomy Building, Thursday 1-2pm (unless otherwise stated)
Thursday June 2nd
Ingrid Lekk (Wilson Lab) Development of left-right asymmetries in the vertebrate brain
Claire Anderson (Stern Lab) A search for new organizers
Thursday June 16th
Pedro Henriques (Bianco Lab NPP)
Nun McHedlishvili (Baum Lab) Microtubule cytoskeleton remodeling during mitotic entry
Monday 20 June, 1.30-4.30pm PhD TALKS
Venue: Room 249, 2nd Floor, Medical Sciences Building, Gower Street
Final Year Students
Host: Michael Duchen
1.30pm Kate Turner
2.00pm Lizzie Yates
2.30pm Alan Greig
First Year Students
Host: Yoshiyuki Yamamoto
3.15pm Alex Henderson
3.30pm Bethan Wolfenden
3.45pm Alessandro Bossio
Final Year Student
Host: Michael Duchen
4.00pm Chris Penny
Hyung Chul (Stern Lab) Different combinations of signal inputs for specific cellular events to establish embryonic axis
Johanna Buchler (Salinas Lab)
Bone Biology Lab
Bone growth and turnover result from the co-ordinated activities of osteoblasts (bone forming cells) and osteoclasts (bone destroying cells). Imbalance in the activities of these cells can lead to the common bone loss disorder osteoporosis.
We are interested in 'fundamental' regulators of the function of osteoclasts and osteoblasts.
Our work falls into 3 main areas:
We discovered that a small pH reduction (acidosis) is necessary to activate osteoclasts to excavate resorption pits on bone surfaces. We found that simultaneously, acidosis prevents mineralisation of newly formed bone by osteoblasts. The impressive responses of bone cells to extracellular pH changes probably represent a primitive ‘failsafe’ to correct systemic acidosis by releasing alkaline bone mineral (animated slide). There is increasing evidence that acidosis due to disease or diet (and perhaps excessive exercise) could play a role in bone loss disorders. Our current research is focused on understanding the mechanisms by which bone cells recognise and respond to pH changes in their environment.
We have found that hypoxia (low oxygen) is a major stimulator of the formation of osteoclasts from precursor cells present in blood and bone marrow. Osteoclast formation – and thus bone resorption – is strongly increased when oxygen is reduced to as little as 1% of the atmospheric level. In contrast to its effect on osteoclasts, we observed that hypoxia strongly blocks the growth and bone-forming capacity of osteoblasts. These observations help to explain why bone is rapidly lost when the blood supply is reduced or disrupted – eg, as a result of inflammation, infection, tumours, fractures, diabetes, chronic obstructive airway diseases and ageing. Our results also explain why bone formation requires a good local blood supply. Significantly, hypoxia also causes tissue acidosis (see above). Our results emphasise the critical role of the vasculature in the maintenance of bone health (animated slide).
ATP and other nucleotides are now recognised to play important and complex regulatory roles as messenger molecules for cell-to-cell communication. In collaboration with Professor Geoff Burnstock FRS, we have studied the actions of nucleotides on bone cell function. We found that numerous P2 nucleotide receptor subtypes are expressed by bone cells and that low concentrations of ATP and ADP stimulate both the formation and activity of osteoclasts, whilst selectively blocking mineralisation of newly formed bone by osteoblasts. Recent results show that skeletal changes can occur when P2 receptors are defective or deficient. This work suggests that the ATP – P2 cell signalling system offers potential for novel bone therapeutics. To help continue this research we are participating in a European consortium.
Current lab members: Isabel Orriss (Research Fellow), Jessal Patel (BBSRC/Unilever PhD Student), Mark Hajjawi (PhD Student), Tim Arnett.
Research support: Arthritis Research Campaign, BBSRC, Unilever Ltd, Amgen Ltd.
Page last modified on 07 oct 13 09:07