Mass Spectrometry
There are two systems available in the Mass Spectrometry suite within the Paul O'Gorman Building - a Kratos AXIMA CFR MALDI-ToF and a Shimadzu LC-MS-ITToF.
Several different protocols can be run on these systems and they fall into 4 main categories:
Contact
Dr Carolyn Hyde
tel: 020 7679 6769
email carolyn.hyde@ucl.ac.uk
1 - Mass Analysis
This can be done on either system.
The AXIMA CFR (a Matrix Assisted Laser Desorption - Ionisation
Time of Flight Instrument) gives monoisotopic resolution of 3000
up to a mass of 6000. It can analyse masses up to approximate
l150,000 Da both in positive and negative mode, very useful for
determiation of intact proteins. Most sample types can be analysed
with the use of the correct matrix.
.
The IT-ToF gives the added dimensions of chromatographic data
and MSn data on each and every peak. It also has the added
feature of formula prediction from a particular mass.
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Please contact Cali to discuss your requirements.
Prices:
| UCL | External | ||
| Sample analysis on AXIMA | 18.25 | 29.20 | per sample |
| Sample analysis on IT-ToF | 83.00 | 132.80 | per hour |
2 - Metabolite Identification
This is run on the It-ToF. There is a dedicated software package to help with this and the ability to do true MSn means that structural information can be elucidated. The accurate masses obtained enables the prediction of formula and this in turn leads to identification of the metabolites from a known parent compound.
Prices:
| UCL | External | |||
| Per Project | 423.20 | 677.12 | standing charge | |
| 230.00 | 369.00 | daily charge | ||
| 40.00 | 64.00 | extra assistance / hour | ||
| one off samples | 83.00 | 132.80 | per hour |
3 - Protein Analysis and Identification
Both systems can be used for this.
One of the commonly used techniques is Peptide Mass Fingerprinting. This utilises the AXIMA CFR and MASCOT. It is especially useful and quick if the protein is pure and you are looking for a final confirmation.
When using the LC-MS-IT-ToF system, the added dimension of chromatographic separation gives more information to be used for the database search. This is enhanced further by the collection of MSn data in both positive and negative modes.
For complex mixtures, it is always best to run the sample down a size exclusion column to separate out the components of the mixture.
Prices:
| UCL | External | |||
| Project cost | standing charge | 423.20 | 677.12 | per project |
| daily charge | 230.00 | 368.00 | per day | |
| extra assistance | 40.00 | 64.00 | per hour | |
| IT-ToF costs | (for one off samples) | 83.00 | 132.80 | per hour |
| AXIMA costs | Peptide Mass Fingerprinting | 40.00 | 64.00 | per sample |
| on membrane digests | 245.00 | 392.00 | per spot |
Downloads and Links:
Matrix Science Website
Gel Band Sensitivity
4 - Tissue Imaging
This exciting new method utilises the AXIMA CFR and measures mass across a tissue sample. It generates a "heat map" for a particular mass, indicating where it is found within the sample. Very useful for the determination of where endogenous proteins are found and where drug molecules end up.
Prices:
| UCL | External | |
| Per image | 245.00 | 392.00 |
