UCL Cancer Institute
Paul O'Gorman Building
72 Huntley Street
London WC1E 6BT
contact@cancer.ucl.ac.uk +44 (0)20 7679 6500
Human
Epigenome Project
Provides
an epigenetic resource of chromosomal DNA methylation
reference profiles in human tissues and cell lines.
The Human Epigenome Project
This part of the Human
Epigenome Project (HEP) was conducted between 1999 and 2006 with the
aim to generate tissue-specific DNA methylation reference
profiles of the human genome.
The chosen approach involved treatment of the genomic DNA with sodium
bisulphite which converts unmethylated cytosines into uracil but does
not affect methylated cytosines. Following PCR amplification and
sequencing of selected amplicons from bisulphite-converted DNA, the
degree of methylation was determined by comparison of the corresponding
signal ratios at CpG dinucleotides, the predominant sites of DNA methylation.
Release 7th Oct 2003
comprised about 135,000 CpG methylation values, obtained from the analysis
of 235 amplicons across the 4Mb major histocompatibility complex on chromosome
6 in 32 samples (derived from 7 different tissues). The
data and results of this pilot study are described in:-
DNA methylation profiling of the human major histocompatibility
complex: a pilot study for the human epigenome project.
Rakyan VK, Hildmann T, Novik KL, Lewin J, Tost J, Cox AV,
Andrews TD, Howe KL, Otto T, Olek A, Fischer J, Gut IG,
Berlin K, Beck S
PLoS Biol. 2004;2;e405.
(pubmed)
Release 26th June 2006 comprised about 1.9
million CpG methylation values, obtained from the analysis of 2,524 amplicons
across chromosomes 6, 20 and 22 in 43 samples (derived from 12 different
tissues). The results of this study are described in:
DNA methylation profiling of human chromosomes 6, 20 and 22.
Eckhardt F, Lewin J, Cortese R, Rakyan VK, Attwood J, Burger
M, Burton J, Cox TV, Davies R, Down TA, Haefliger C, Horton
R, Howe K, Jackson DK, Kunde J, Koenig C, Liddle J, Niblett
D, Otto T, Pettett R, Seemann S, Thompson C, West T, Rogers
J, Olek A, Berlin K, Beck S
Nat Genet. 2006;.
(pubmed)
Stephan Beck, PhD FMedSci
Prof. of Medical Genomics
UCL Cancer Institute
University College London
Paul O’Gorman Building
72 Huntley Street
London WC1E 6BT, UK
Tel: +44-20-7679-0964
s.beck@ucl.ac.uk
contact@cancer.ucl.ac.uk
+44 (0)20 7679 6500
