Biochemical Engineering


Dr Pierre-Yves Colin

Post-doctoral Research Associate


  • PhD in Biochemistry, University of Cambridge - UK (2011-2015)
  • MSc in Biochemical Engineering, INSA Toulouse – France (2009-2011)

Research interests

I take advantage of ultrahigh throughput screening and selection strategies (e.g. microfluidic droplets, FACS) to identify new sequence-function relationships in proteins. I previously used this approach in the context of functional metagenomics to isolate rare hits from environmental gene libraries. I now use libraries of enzyme variants and directed evolution to identify and engineer fit biocatalysts for industrial applications.   

Selected publications

  • Colin, P.Y., Kintses, B., Gielen, F., Miton, C., Fischer, G. Mohamed, M., Hyvonen, M., Morgavi, D.P., Janssen, D.B. and Hollfelder, F. Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nat. Comm. 2015, 6: 10008
  • Colin, P.Y., Zinchenko, A., Hollfelder, F. (2015). Enzyme Engineering in Biomimetic Compartments. Curr. Opin. Struct. Biol. 2015, 33: 42-51
  • van Vliet, L.D., Colin P.Y., Hollfelder F. Bioinspired genotype-phenotype linkages: mimicking cellular compartmentalisation for the engineering of functional proteins. Interface focus. 2015, 5: 20150035
  • Zinchenko, A., Devenish, S.R., Kintses, B., Colin, P.Y., Fischlechner, M., and Hollfelder, F. (2014). One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution. Anal. Chem. 2014, 86: 2526-2533.