Downstream Processing: From Cell to Column: Integrated Solutions for Rapid Bioprocessing of Biologics 

Learn how to select the best recovery options for your bioprocess

Autumn 2015

This module focuses on the biological product recovery stage and, through a series of lectures, case studies and pilot plant practical sessions, will enable you to:

  • Understand the principles of all the major recovery operations: Centrifugation, Membrane Systems, Filtration, Cell Disruption, Precipitation, Flocculation
  • Compare these unit operations for suitability within the process and study them in our unique pilot plant
  •  Determine the most appropriate equipment for separations and specify the right operating conditions
  • Learn to use Ultra Scale Down tool for prediction of large scale separation performance
  • Understand the interaction among fermentation, recovery and purification and learn the integrated solutions for bioprocess development

Who should attend?

This module is designed for scientists and engineers involved in design, development and operation of primary recovery steps as well as associated steps in upstream and purification processes.

  • Bioprocess developer
  • R&D scientist
  • Manufacturing technologist
  • Manufacturing operational manager


Day 1

• Introduction to module
• Principles of precipitation and design of precipitation reactors
• Centrifugation
• Ultra scale down centrifugation
• Case study: design of precipitation reactor

Day 2

• Pilot-plant studies: centrifugation
• Intracellular product release
• Filtration
• Microfiltration and ultrafiltration
• Ultra scale down microfiltration

Day 3

• Pilot-plant studies: microfiltration
• Bioprocess applications of centrifuges
• From lab scale to manufacturing: microfiltration technology for product recovery and purification
• Use of adsorptive depth filtration in biopharmaceutical manufacturing

Day 4

• Simulation studies: the role of microfiltration in whole bioprocess studies
• Cell engineering to ease primary recovery
• Use flocculation to remove key mpurities to prepare feed material for purification
• Primary recovery process development with an integrated approach
• Viral product recovery in vaccine processing

Teaching Staff

Dr Yuhong Zhou - Module Leader
Prof Mike Hoare, UCL
Prof Eli Keshavarz-Moore, UCL
Prof Nigel Titchener-Hooker, UCL
Dr Darren Nesbeth, UCL

Invited Expert Speakers

Colin Day, Millipore
Klaus Mannweiler, Westfalia
Aloke Dey-Chowdhury, Pall Life Sciences

A networking dinner will be held on the first evening.

Module cost TBC.

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Page last modified on 26 mar 15 14:10