Post-doctoral Research Associate
Phone: +44(0)20 7679 3316 (X33316 Internally)
Licenciate in Chemistry, Universidade Nova de Lisboa
PhD Bioengineering, MIT-Portugal programme, Universidade Nova de Lisboa
Bioprocess engineering of stem cells.
The differentiation of stem to adult tissue of clinical relevance is a goal pursued by many academic groups and industrial stakeholders. While the biology underlying stem cell differentiation is progressing and several clinical trials are already taking place worldwide, there is the need to establish economically viable and regulatory compliant stem cell bioprocesses.
Rui’s research is focused i) on the adaptation of adapt current stem cell differentiation protocols to the large scale production of functional adult cells and ii) the creation of Point of Care (POC) solutions which enable clinical staff to perform stem cell therapies in clinical settings without worrying about cell processing, viability or differentiation.
Human liver cell spheroids in extended perfusion bioreactor culture for repeated dose drug testing. Tostões RM, Leite SB, Serra, Margarida; Jensen, Janne; Björquist, Petter; Carrondo MJ, Brito, Catarina; Alves PM. Hepatology, April 2012.
Perfusion of 3D encapsulated hepatocytes-A synergistic effect enhancing long-term functionality in bioreactors. Tostões RM, Leite SB, Miranda JP, Sousa M, Wang DI, Carrondo MJ, Alves PM Biotechnology & Bioengineering, vol. 108, Issue 1, Jan 2011, 41-49.
Merging bioreactor technology with 3D hepatocyte-fibroblast culturing approaches: Improved in vitro models for toxicological applications. Leite SB, Teixeira AP, Miranda JP, Tostões RM, Clemente JJ, Sousa MF, Carrondo MJ, Alves PM. Toxicol In Vitro. 2011 Feb 16.
Improving expansion of pluripotent human embryonic stem cells in perfused bioreactors through oxygen control.Serra M, Brito C, Sousa MF, Jensen J, Tostões R, Clemente J, Strehl R, Hyllner J, Carrondo MJ, Alves PM. J Biotechnol. 2010 Aug 2;148(4):208-15.
Extending hepatocyte functionality for drug-testing applications using high-viscosity alginate-encapsulated three-dimensional cultures in bioreactors. Miranda JP, Rodrigues A, Tostões RM, Leite S, Zimmerman H, Carrondo MJ, Alves PM. Tissue Eng Part C. 2010 Dec;16(6):1223-32.
Differential requirements of a mitotic acetyltransferase in somatic and germ line cells. Pimenta-Marques A, Tostões R, Marty T, Barbosa V, Lehmann R, Martinho RG, Developmental Biology, vol. 323, Issue 2, 15 November 2008, Pages 197-206.
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