Literature references :
Nielsen PA, Mandel U, Therkildsen MH, Clausen H.  Differential expression of human high-molecular-weight salivary mucin (MG1) and low-molecular-weight salivary mucin (MG2).
J Dent Res 1996 Nov;75(11):1820-6

Isotype : IgG1

Origin, source : Mice were immunised with the partially deglycosylated MG1 salivary mucin.

Specificity : The antibody reacts with the partially deglycosylated MG1 mucin.  PANH2 recognises a protein epitope within MG1 which is partially masked in the fully glycosylated secreted mucin.

Cross-reactivity : The PANH2 monoclonal antibody does not react with Tn antigen on ELISA or on Western blots.

Binding Assays : The hybridomas were screened on ELISA assays against the partially deglycosylated MG1 mucin.

Immunoblotting : With whole saliva and purified MG1 PANH2 stains a high molecular weight smear; after deglycosylation the smear extends further down the gel. Pronase treatment eliminates the antibody reactivity.

Immunohistochemistry:
On frozen tissues
 

Positive reactivity	Negative reactivity
Mucous cells (staining of the basal part of the cytoplasm)in submandibular, sublingual and small salivary glans of the lip and palate	Serous cells in submandibular, sublingual and small salivary glans of the lip and palate Duct cells intercalated ducts, striated ducts and excretory ducts

Experiments performed in Jacques Bara's Lab:

 The PMH2 antibody did not work on ethanol fixed tissues.
 

Other literature references (using the antibody) :
Croce MV, Colussi AG, Price MR, Segal-Eiras A  Identification and characterization of different subpopulations in a human lung  adenocarcinoma cell line (A549).
Pathol Oncol Res 1999;5(3):197-204

Note : Made in H.Clausen Laboratory